Product Name :
Rabbit anti-Nestin Polyclonal Antibody
Synonym :
NES
Host :
Rabbit
Species Reactivity:
Human
Specificity :
Nestin Antibody detects endogenous levels of total Nestin.
Predicted Reactivity:
Applications :
WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Immunogen:
A synthesized peptide derived from Human NES(Accession P48681), corresponding to amino acid residues V1477-S1498.
Concentration :
1mg/ml
Purification :
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality:
Monoclonal Antibody
Storage Temp.:
Store at -20 ° C for one yearAvoid repeated freeze/that cycles
Research areas :
Background :
Nestin is a member of the intermediate filament family, which is expressed in a variety of stem or progenitor cells as well as in several types of malignancies. It was originally described in neuroepithelial stem cells of developing and adult brains and, since then, its expression has been described in many types of stem/progenitor cells in tissues such as the bone marrow, heart, skeletal muscle, pancreas, testis, and hair. In these and other sites, nestin might have a role in active proliferation, wound healing, and tissue regeneration [PMID: 34943921].
UniProt :
P48681
Additional information:
Product Details FAQ Citations(2) Video Pictures Documents |Overview |Synonym NES |Host Rabbit |Specificity Nestin Antibody detects endogenous levels of total Nestin. |Species Reactivity Human |Applications WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 |Immunogen A synthesized peptide derived from Human NES(Accession P48681), corresponding to amino acid residues V1477-S1498. |Properties |Form Liquid |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Monoclonal Antibody |Clone R112 |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background Nestin is a member of the intermediate filament family, which is expressed in a variety of stem or progenitor cells as well as in several types of malignancies. It was originally described in neuroepithelial stem cells of developing and adult brains and, since then, its expression has been described in many types of stem/progenitor cells in tissues such as the bone marrow, heart, skeletal muscle, pancreas, testis, and hair. In these and other sites, nestin might have a role in active proliferation, wound healing, and tissue regeneration [PMID: 34943921]. |tissuespecificity CNS stem cells. |Post-translational modifications Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity). |Cellular localization Cytoskeleton;Extracellular region or secreted; |UniProt P48681 | Western blot analysis Nestin using MCF7 whole cell lysates |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-Nestin Recombinant Monoclonal Antibody(R112)
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
2431972-52-8 Protocol 171596-29-5 Biological Activity PMID:31194392 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
