Name :
MERTK/Mer Protein
Description :
MER tyrosine kinase (MERTK) encodes a surface receptor localized at the apical membrane of the retinal pigment epithelium. It plays a critical role in photoreceptor outer segment internalization prior to phagocytosis. Mutations in MERTK have been associated with severe autosomal recessive retinal dystrophies in the RCS rat and in humans.
Species :
Human
Uniprotkb :
HEK293
Tag :
C-His
Synonyms :
Tyro12, MGC133349, c-mer, MERTK, c-Eyk, RP38, MER
Construction :
Recombinant Human MERTK/Mer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala21-Ile505.[Accession |Q12866-1]
Protein Purity :
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight :
The protein has a predicted MW of 53.73 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.
Endotoxin :
Less than 1EU per μg by the LAL method.
Formulatione :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage :
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
MER tyrosine kinase (MERTK) encodes a surface receptor localized at the apical membrane of the retinal pigment epithelium. It plays a critical role in photoreceptor outer segment internalization prior to phagocytosis. Mutations in MERTK have been associated with severe autosomal recessive retinal dystrophies in the RCS rat and in humans.
References and Literature :
1. Wu J, et al. MerTK as a therapeutic target in glioblastoma. Neuro Oncol. 2018 Jan 10;20(1):92-102. doi: 10.1093/neuonc/nox11 PMID: 28605477; PMCID: PMC5761530.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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