In injury. (C) ARKO mice show afterafter TBI.The quantitative data of GFAP level at 4 hat four h following brain injury. (C) ARKO mice show TBI-induced GFAP expression enhancement compared 24 h right after TBI. (D) TBI. (D) QuantiTBI-induced GFAP expression enhancement compared with WTwith WT 24 h soon after Quantitative information tative information of GFAPhlevel at 24 hTBI. All information are presented as the mean normal standard erof GFAP level at 24 following following TBI. All data are presented as the imply error. NS, no ror. NS, no considerable distinction; p 0.01, and p 0.001; n = three in each group. substantial difference; p 0.01, and p 0.001; n = three in each and every group.Molecules 2021, 26, 6250 Molecules 2021, 26, x FOR PEER REVIEW5 of 16 five ofFigure three. Androgen receptor knockout increases the TBI-induced GFAP expression about thethe Figure three. Androgen receptor knockout increases the TBI-induced GFAP expression about cortical injury site. (A) Illustrations in the regions of interest (white places) the mice brain right after TBI cortical injury website. (A) Illustrations with the regions of interest (white areas) ofof the mice brain after TBI are shown in left panel. WT ARKO mice have been performed with TBI or sham, and after that stained are shown in left panel. WT and and ARKO mice had been performed with TBI or sham, and after that stained with immunofluorescence of GFAP. The GFAP cells had been indicated by white white arwith immunofluorescence of GFAP. The GFAP constructive good cells have been indicated byarrowhead. rowhead. ARKO mice showed the cells of GFAP of GFAP expression. Blue colour, DAPI (four,6ARKO mice showed the increasingincreasing cellsexpression. Blue colour, DAPI (4 ,6-diamidino-2diamidino-2-phenylindole); red colour, GFAP. (Images: x200 magnification of the ipsilateral plus the phenylindole); red color, GFAP. (Pictures: x200 magnification with the ipsilateral and also the contralateral contralateral hemispheres; scale bar = one hundred m) (B) The intensity of GFAP immunoreactive level hemispheres; scale bar = 100 ) (B) The intensity of GFAP immunoreactive level with normalized with normalized intensity fluorescence unit within the 4 experimental groups is presented. (C) The intensity fluorescence positive cells counterstained with DAPI in the 4 experimental groups is percentage of GFAP unit in the four experimental groups is presented. (C) The percentage of GFAP good cells JPH203 site counterstainedof GFAP at the corticalexperimental groups is presented. The expression presented. The expression with DAPI inside the 4 injury web page was calculated from six distinctive of GFAP levels.corticalwild-type sham; WT-T, wild-type with TBI;
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