Matched the identified proteins using the genome of L. vannamei, E.
Matched the recognized proteins using the genome of L. vannamei, E. sinensis, P. trituberculatus, and drosophila fly, respectively. Commonly speaking, the unigenes of M. nipponense transcriptome showed the highest sequence identities with that of E. sinensis. Gene Ontology (GO) and Cluster of Orthologous Groups (COG)analysis aimed to supply a structured vocabulary to describe gene items. A total of 19,673 (39.76 ) unigenes have been assigned for the GO database comprised of 52 functional groups (Fig. two). The number of unigenes in every functional group ranged from 1 to ten,057. A total of 13,395 (27.07 ) unigenes have been hugely matched with known proteins within the COG database that had been classified into 25 functional groups (Fig. three). The amount of unigenes in each and every functional group ranged from 1 to 6793. Kyoto Encyclopedia of Genes and Genomes (KEGG) evaluation aimed to reveal the regulatory relationship in between unigenes in the long-read transcriptome (www.kegg.jp/kegg/kegg1.html). A total of 18,618 (36.72 ) unigenes had been very matched recognized genes within the KEGG database, mapped onto 264 metabolic pathways.Long-read transcriptome. A total of 22.83 GBs of clean information had been generated within the long-read transcrip-Identification of differentially expressed genes. Differentially expressed genes (DEGs) had been iden-tified, applying the criterion of 2.0 as up-regulatory genes and 0.5 as GLP Receptor Agonist Source down-regulatory genes, and using a P worth 0.05. A total of 1319 DEGs had been identified among CG and SS, like 713 up-regulated genes and 606 down-regulated genes. A total of 2092 DEGs had been identified between SS and DS, including 1036 up-regudoi/10.1038/s41598-021-99022-4 three Vol.:(0123456789)Scientific Reports |(2021) 11:19855 |www.nature.com/scientificreports/Figure 3. Cluster of orthologous groups (COG) classification of putative proteins. lated genes and 1056 down-regulated genes. A total of 4351 DEGs have been identified among CG and DS, like 2163 up-regulatory genes and 2188 down-regulatory genes. KEGG evaluation revealed that Cell cycle, Cellular Senescence, Oxidative Phosphorylation, Glycolysis/Gluconeogenesis and Steroid Hormone Biosynthesis had been the key enriched metabolic pathways in all of these three comparisons. A total of 15 DEGs have been selected from these enriched metabolic pathways, which are listed in Table 1. These genes have been differentially expressed in a minimum of two of your three comparisons. Cyclin B3, MAD2A, Pololike kinase 1, Cyclin A, cyclin-dependent kinase two (Cdk2) and Cyclin B have been identified within the metabolic pathways of Cell cycle and Cellular senescence, which had been differentially expressed in all 3 comparisons. Succinate dehydrogenase complicated iron sulfur subunit B Gene (SDHB), Cytochrome c oxidase assembly protein COX11 and Cytochrome c oxidase subunit 7A1 were chosen from the metabolic pathways of Oxidative Phosphorylation. Acetyl-coenzyme A synthetase 2-like, Fructose-bisphosphate aldolase and Alcohol dehydrogenase class-3 were differentially expressed in the metabolic pathways of Glycolysis/Gluconeogenesis. T-type calcium channel Formulation Estrogen Sulfotransferase, 3 beta-hydroxysteroid dehydrogenase and HSDL1 have been identified in the metabolic pathways of Steroid Hormone Biosynthesis.qPCR verification. qPCR analysis was used to verify the expressions of significant DEGs within the androgenicgland from the CG, SS, and DS prawns. We selected ten out of 15 DEGs to verify the accuracy of RNA-seq. The qPCR analysis showed the identical expression pattern as the RNA-seq (Fig. four). Six DEGs in the metabolic pa.
