Product Name :
E.coli SS320
Applications:
phage display system
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The E. coli strain SS320 is a non-amber suppressor strain (sometimes called MC1061F′) prepared as high transformation efficiency (≥ 1 × 109 cfu/μg) chemically competent cells for phage display library screening. The SS320 strain harbors an episome that contains the F′ pilus that is required for bacteriophage entry to allow amplification of phage libraries. This episome also contains the tetracycline resistance gene, so cells should be grown in the presence of tetracycline to maintain the F′ pilus. E. coli strain SS320 are suitable for protein expression, general cloning, blue/white screening, M13 phage work and phage display protein expression.IntroductionStrains Resistance TetCulture Medium LBCondition 37℃ ,under the aerobic conditionsThe E. coli strain SS320 is a non-amber suppressor strain (sometimes called MC1061F′) prepared as high transformation efficiency (≥ 1 × 109 cfu/μg) chemically competent cells for phage display library screening.{{697235-38-4} site|{697235-38-4} Protocol|{697235-38-4} Formula|{697235-38-4} custom synthesis} The SS320 strain harbors an episome that contains the F′ pilus that is required for bacteriophage entry to allow amplification of phage libraries. This episome also contains the tetracycline resistance gene, so cells should be grown in the presence of tetracycline to maintain the F′ pilus.{{2144751-78-8} medchemexpress|{2144751-78-8} Purity & Documentation|{2144751-78-8} Formula|{2144751-78-8} supplier} E.PMID:30485004 coli strain SS320 are suitable for protein expression, general cloning, blue/white screening, M13 phage work and phage display protein expression.GenotypehsdR mcrB araD139 Δ(araABC-leu)7679ΔlacX74 galUgalK rpsL thi[F´ proAB+lacIqlacZΔM15 Tn10 (tetr)]
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